The FLIPR assay was executed on FDSS/µCell with ligand addition at 10?s with the binding assay and mAb1/isotype addition at 10?s accompanied by hCCL1 addition at three hundred?s for your Competitors binding assay, with a complete checking of 500?s. The excitation and emission wavelengths ended up set at 485?nm and 525?nm, respectively. Following t